Immunohistochemistry:Article Title: Arginine reprograms metabolism in liver cancer via RBM39.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ARG1 GeneTex Cat#109242 Rabbit anti-AGMAT Novus Biological Cat#1-82080 Rabbit anti-CPS1 abcam Cat#129076 Mouse anti-OTC SantaCruz Biotech Cat#515791 Mouse anti-ASS1 SantaCruz Biotech Cat#365475 Mouse anti-ASL SantaCruz Biotech Cat#166787 Rabbit anti-SLC7A1 abcam Cat#37588 Rabbit anti-SLC7A6 MyBiosource Cat#7103267 Rabbit anti-SLC7A7 Epigentek Cat#A68118-020 Rabbit anti-ODC GeneTex Cat#54600 Rabbit anti-SRM ThermoFisher Scientific Cat#PA5-31341 Mouse anti-SMS SantaCruz Biotech Cat376294 Rabbit anti-SAT1 Novus Biological Cat#110-41622 Mouse anti-PAOX SantaCruz Biotech Cat#166185 Rabbit anti-SMOX abcam Cat# 213631 Rabbit anti-AKT Cell Signaling Cat#4685 Rabbit anti-AKT-pS473 Cell Signaling Cat#9217 Rabbit anti-Calnexin Enzo Life Sciences Cat#ADI-SPA-860-F Mouse anti-Actin Millipore Cat#MAB1501 Rabbit anti-ASNS GeneTex Cat#30068 Rabbit anti-PSAT1 GeneTex Cat#633629 Rabbit anti-PSPH GeneTex Cat#33442 Mouse anti-NNMT abcam Cat#119758 Rabbit anti-S6 Cell Signaling Cat#2217 Rabbit anti-S6-pS240,244 Cell Signaling Cat#5364 Rabbit anti-RBM39 Sigma Cat#HPA001591 Rabbit anti-RBM39 Bethyl Laboratories Cat#A300-291A Mouse anti-FLAG M2 Sigma Cat#F1804 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-Strep Invitrogen Cat#MA5-37747 Mouse anti-eIF2a Cell Signaling Cat#2103 Rabbit anti-eIF2a-pS51 Cell Signaling Cat#3957 Rabbit anti-SESN2 abcam Cat#ab178518 Rabbit anti-AGMAT (for IHC) Sigma Cat#PA5-55311 Mouse anti-CASTOR1 SantaCruz Biotech Cat#377114 Mouse anti-H3 Cell Signaling Cat#14269 Mouse anti-GAPDH SantaCruz Biotech Cat#365062 Biological samples HCC patient tumor and non-tumor tissues University Hospital Basel N/A HCC patient-derived organoids University Hospital Basel N/A Chemicals, peptides, and recombinant proteins Indisulam Sigma Cat#SML1225 Indisulam MedKoo Biosciences Cat#201540 (Continued on next page) e1 Cell 186, 5068–5083.e1–e12, November 9, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Arginine-coupled agarose beads gbiosciences Cat#GENO786-1361 L-leucine-coupled agarose beads gbiosciences Cat#GENO786-1370 Critical commercial assays Total Polyamine Assay Kit BioVision Cat#K475 L-arginine ELISA kit MyBiosource Cat#MBS728648-96 Nano-Glo dual luciferase reporter assay kit Promega Cat#N1610 Deposited data HCC patients’ RNA sequencing data Ng et al., 202250 EGAS00001005073, EGAS00001005074 HCC patients’ proteome data Ng et al., 202250 PRIDE (PXD025705, PXD025836) Mouse RNA sequencing data Dimitrakopoulos et al., 202125 SRP156216 Mouse proteome data Dimitrakopoulos et al., 202125 https://github.com/cbgethz/netics/tree/ master/mouse_data Mouse and HCC patients’ metabolomics This paper MASSIVE: MSV000092406 RNA sequencing data of ARG1/AGMAT-expressing (and control) and RBM39-depleted (and control) SNU-449 cells This paper GEO: PRJNA940402 Mouse, HCC patients, and SNU-449 proteome data of pulldowns using Leu- and Arg-coupled agarose beads This paper MASSIVE: MSV000091516 Experimental models: Cell lines SNU-182 Diego Calvisi (University of Sassari, Italy) N/A SNU-449 Diego Calvisi (University of Sassari, Italy) N/A HLE Diego Calvisi (University of Sassari, Italy) N/A PLC Diego Calvisi (University of Sassari, Italy) N/A Hep40 Diego Calvisi (University of Sassari, Italy) N/A Hep3B Diego Calvisi (University of Sassari, Italy) N/A Huh6 Diego Calvisi (University of Sassari, Italy) N/A Huh7 Diego Calvisi (University of Sassari, Italy) N/A HepG2 Diego Calvisi (University of Sassari, Italy) N/A SNU-475 Gerhard Christofori (University of Basel, Switzerland) N/A SNU-423 Gerhard Christofori (University of Basel, Switzerland) N/A Huh1 Gerhard Christofori (University of Basel, Switzerland) N/A HEK293 ATCC CRL-1573 HEK239T ATCC CRL-3216 SNU-449 control This paper N/A SNU-449 ARG1 This paper N/A SNU-449 AGMAT This paper N/A SNU-449 ARG1/AGMAT This paper N/A SNU-449 ASS1-FLAG This paper N/A SNU-449 ARG1/AGMAT +ASS1 This paper N/A SNU-449 ARG1/AGMAT +3xHA-ASS1 This paper N/A Huh7 sgCtrl This paper N/A Huh7 sgARG1 This paper N/A Huh7 sgAGMAT This paper N/A SNU-449 ARG1/AGMAT +control (for ASNS) This paper N/A (Continued on next page) ll OPEN ACCESS Cell 186, 5068–5083.e1–e12, November 9, 2023 e2 Article
Recombinant:Article Title: Arginine reprograms metabolism in liver cancer via RBM39.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ARG1 GeneTex Cat#109242 Rabbit anti-AGMAT Novus Biological Cat#1-82080 Rabbit anti-CPS1 abcam Cat#129076 Mouse anti-OTC SantaCruz Biotech Cat#515791 Mouse anti-ASS1 SantaCruz Biotech Cat#365475 Mouse anti-ASL SantaCruz Biotech Cat#166787 Rabbit anti-SLC7A1 abcam Cat#37588 Rabbit anti-SLC7A6 MyBiosource Cat#7103267 Rabbit anti-SLC7A7 Epigentek Cat#A68118-020 Rabbit anti-ODC GeneTex Cat#54600 Rabbit anti-SRM ThermoFisher Scientific Cat#PA5-31341 Mouse anti-SMS SantaCruz Biotech Cat376294 Rabbit anti-SAT1 Novus Biological Cat#110-41622 Mouse anti-PAOX SantaCruz Biotech Cat#166185 Rabbit anti-SMOX abcam Cat# 213631 Rabbit anti-AKT Cell Signaling Cat#4685 Rabbit anti-AKT-pS473 Cell Signaling Cat#9217 Rabbit anti-Calnexin Enzo Life Sciences Cat#ADI-SPA-860-F Mouse anti-Actin Millipore Cat#MAB1501 Rabbit anti-ASNS GeneTex Cat#30068 Rabbit anti-PSAT1 GeneTex Cat#633629 Rabbit anti-PSPH GeneTex Cat#33442 Mouse anti-NNMT abcam Cat#119758 Rabbit anti-S6 Cell Signaling Cat#2217 Rabbit anti-S6-pS240,244 Cell Signaling Cat#5364 Rabbit anti-RBM39 Sigma Cat#HPA001591 Rabbit anti-RBM39 Bethyl Laboratories Cat#A300-291A Mouse anti-FLAG M2 Sigma Cat#F1804 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-Strep Invitrogen Cat#MA5-37747 Mouse anti-eIF2a Cell Signaling Cat#2103 Rabbit anti-eIF2a-pS51 Cell Signaling Cat#3957 Rabbit anti-SESN2 abcam Cat#ab178518 Rabbit anti-AGMAT (for IHC) Sigma Cat#PA5-55311 Mouse anti-CASTOR1 SantaCruz Biotech Cat#377114 Mouse anti-H3 Cell Signaling Cat#14269 Mouse anti-GAPDH SantaCruz Biotech Cat#365062 Biological samples HCC patient tumor and non-tumor tissues University Hospital Basel N/A HCC patient-derived organoids University Hospital Basel N/A Chemicals, peptides, and recombinant proteins Indisulam Sigma Cat#SML1225 Indisulam MedKoo Biosciences Cat#201540 (Continued on next page) e1 Cell 186, 5068–5083.e1–e12, November 9, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER L-Arginine-coupled agarose beads gbiosciences Cat#GENO786-1361 L-leucine-coupled agarose beads gbiosciences Cat#GENO786-1370 Critical commercial assays Total Polyamine Assay Kit BioVision Cat#K475 L-arginine ELISA kit MyBiosource Cat#MBS728648-96 Nano-Glo dual luciferase reporter assay kit Promega Cat#N1610 Deposited data HCC patients’ RNA sequencing data Ng et al., 202250 EGAS00001005073, EGAS00001005074 HCC patients’ proteome data Ng et al., 202250 PRIDE (PXD025705, PXD025836) Mouse RNA sequencing data Dimitrakopoulos et al., 202125 SRP156216 Mouse proteome data Dimitrakopoulos et al., 202125 https://github.com/cbgethz/netics/tree/ master/mouse_data Mouse and HCC patients’ metabolomics This paper MASSIVE: MSV000092406 RNA sequencing data of ARG1/AGMAT-expressing (and control) and RBM39-depleted (and control) SNU-449 cells This paper GEO: PRJNA940402 Mouse, HCC patients, and SNU-449 proteome data of pulldowns using Leu- and Arg-coupled agarose beads This paper MASSIVE: MSV000091516 Experimental models: Cell lines SNU-182 Diego Calvisi (University of Sassari, Italy) N/A SNU-449 Diego Calvisi (University of Sassari, Italy) N/A HLE Diego Calvisi (University of Sassari, Italy) N/A PLC Diego Calvisi (University of Sassari, Italy) N/A Hep40 Diego Calvisi (University of Sassari, Italy) N/A Hep3B Diego Calvisi (University of Sassari, Italy) N/A Huh6 Diego Calvisi (University of Sassari, Italy) N/A Huh7 Diego Calvisi (University of Sassari, Italy) N/A HepG2 Diego Calvisi (University of Sassari, Italy) N/A SNU-475 Gerhard Christofori (University of Basel, Switzerland) N/A SNU-423 Gerhard Christofori (University of Basel, Switzerland) N/A Huh1 Gerhard Christofori (University of Basel, Switzerland) N/A HEK293 ATCC CRL-1573 HEK239T ATCC CRL-3216 SNU-449 control This paper N/A SNU-449 ARG1 This paper N/A SNU-449 AGMAT This paper N/A SNU-449 ARG1/AGMAT This paper N/A SNU-449 ASS1-FLAG This paper N/A SNU-449 ARG1/AGMAT +ASS1 This paper N/A SNU-449 ARG1/AGMAT +3xHA-ASS1 This paper N/A Huh7 sgCtrl This paper N/A Huh7 sgARG1 This paper N/A Huh7 sgAGMAT This paper N/A SNU-449 ARG1/AGMAT +control (for ASNS) This paper N/A (Continued on next page) ll OPEN ACCESS Cell 186, 5068–5083.e1–e12, November 9, 2023 e2 Article
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